HPLC
High-Performance Liquid Chromatography (HPLC) is one of the most important methods used in analytical chemistry. It has the ability to separate, identify, and quantify the compounds present in any sample. It is an instrument that relies on the physical separation of the active substance through two phases: one stationary and the other mobile. This process is represented by the appearance of a peak, whose enclosed area is measured and compared with the peak area of a known standard solution.
HPLC surpasses GC in its ability to handle non-volatile substances, including inorganic ions and thermally unstable materials.
Applications:
It is used to detect impurities in pharmaceutical industries.
It is used to determine the initial concentrations of trace elements.
Chromatography of composite materials.
Ion-exchange chromatography for proteins.
High-pH ion-exchange chromatography for carbohydrates and low-acidity sugars.
